Part:BBa_K3726048:Design
BOH_2_B_LV1
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1751
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 431
Illegal NheI site found at 454
Illegal NheI site found at 757
Illegal NheI site found at 985
Illegal PstI site found at 1751 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2096
Illegal BglII site found at 2213
Illegal BglII site found at 4632
Illegal XhoI site found at 3316
Illegal XhoI site found at 4159 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1751
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1751
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part 5'Con3 “BBa_K2560067” in its upstream region that will allow the assembly of a Lv2 construct following the marburg collection standard. And by the part “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) In its downstream region, which is a homology region for homologous recombination within the genome of PCC 11801.
Source
This construct has been made by golden gate reaction.
References
X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.